Key features and details | |
Cat. No. | MABL-2790 |
Name | Anti-Rad51B mAbs |
Clone No. | AFD- 1E11/6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB |
Species Reactivity | Hamster, Human |
Basic Information | |
Specificity | This antibody recognises human and hamster Rad51B, paralog of Rad51 which is an E. coli RecA homologue. It is invloved in the homologous recombination repair (HRR) pathway which fixes double- stranded breaks. |
Alternative Name | DNA repair protein RAD51 homolog 2; R51H2; RAD51 homolog B; RAD51- like protein 1; REC2; RAD51L1; 1E11 |
UniProt | O15315 |
Immunogen | This antibody was raised against His-tagged human Rad51B, overexpressed in E. coli and purified on a talon affinity column under denaturing conditions, followed by gel purification using SDS-PAGE. |
Application Notes | WB: Rad51B 1E11/6 antibody (1E11) detected Rad51B in a material from V79 cells used as a control in Western blot analysis of immunoprecipitated Rad51 complexes (French et al., 2002). |
Antibody First Published | French et al. Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability. J Biol Chem. 2002 May 31;277(22):19322-30. Epub 2002 Mar 23. PMID:11912211 |
Note on publication | This article describes the earliest use of Rad51B 1E11/6 (1E11/6) antibody to detect Rad51B protein via Western blotting. However, the exact process of generation of this antibody is described in the following paper (where similar antibodies were generated): Masson, J. Y., Stasiak, A. Z., Stasiak, A., Benson, F. E., & West, S. C. (2001). Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proceedings of the National Academy of Sciences, 98(15), 8440-8446. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
