Key features and details | |
Cat. No. | MABL-2821 |
Name | Anti-Reticulon-1C mAbs |
Clone No. | AFD- RNL-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IF, IHC |
Species Reactivity | Rat, Swine, Human |
Basic Information | |
Specificity | The antibody is specific for reticulon-1C. It recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C). |
Alternative Name | NSP-C; Reticulon-1; Neuroendocrine-specific protein |
UniProt | Q16799 |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C. |
Application Notes | The specificity of the original format of the antibody for NSP-C was established in an ELISA assay. Immunofluorescence analysis of COS-1 cells, transfected with NSP-C cDNA, showed staining of the endoplasmic reticulum with the antibody. The antibody detected NSP-C by western blot analysis (Senden et al., 1996; PMID: 8900485). This antibody was used for immunohistochemistry for the detection of NSP-C on lung carcinoid, atypical carcinoid of the lung, SCLC and squamous cell carcinoma (Senden et al., 1997; PMID: 9227337). |
Antibody First Published | Senden et al. Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A Eur J Cell Biol. 1996 Mar;69(3):197-213. PMID:8900485 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
