Anti-Rhesus D antigen mAbs

Cat. No.

MABL-2824

Name

Anti-Rhesus D antigen   mAbs

Clone No.

AFD- D7C2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ADCC, FC

Species Reactivity

Human

Basic Information

Specificity

Alternative Name

CD240D; RHXIII; Rh polypeptide 2; RhPII

UniProt

Q02161

Immunogen

A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes   were isolated and transformed with 1 ml of culture supernatant of the EBV   marmoset B96-8 cell line.

Application Notes

FC confirmed the binding of D7C2 to Rh D+   red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2   showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been   expressed recombinantly using a baculovirus-insect cell expression system   (Edelman et al, 1997).

Antibody First   Published

Edelman et al, 1997. Obtaining a functional   recombinant anti-rhesus (D) antibody using the baculovirus-insect cell   expression system. Immunology. 1997 May; 91(1): 13–19. PMID:9203960

Note on publication

Describes the original production of the   antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.