Anti-Rad51 mAbs

Cat. No.

MABL-2789

Name

Anti-Rad51 mAbs

Clone No.

AFD- 3C10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF, IHC

Species Reactivity

Cow, Pig, Human

Basic Information

Specificity

This antibody recognises RAD51 (and might   also recognise hDmc1 (Masson et al., 1999)). RAD51 is a eukaryotic homologue   of E. coli RecA. It is an important part of the homologous recombination DNA   repair pathway during the homologous strand exchange stage.

Alternative Name

DNA repair protein RAD51 homolog 1; HsRAD51;   hRAD51; RAD51 homolog A; BRCC5; HsT16930; RAD51 homolog; homolog of RecA, E.   coli; RecA-like protein; RAD51ABRCA1/BRCA2-containing complex, subunit 5;   Ab1; Ab-1; Rad51 Ab1; 51RAD01

UniProt

Q06609

Immunogen

This antibody was raised against the   recombinant human RAD51 protein.

Application Notes

WB: Anti-Rad51 3C10 antibody was used to   visualise Rad51 via Western blot after previous immunoprecipitation with   different antibodies in experiments analysing the interactions between Rad51   and DMC1 (Masson et al., 1999). IF: 3C10 antibody was used to visualise Rad51   on chromatin in endopolyploid tumour cells in experiments investigating the   role of another protein - REC8 in chromosome segregation after radiation   (Erenpreisa et al., 2009). IHC: This antibody was also used to immunohistochemically stain biopsy and resected specimens of esophagus and   squamous cell carcinoma of the esophagus (Nakanoko et al., 2014).

Antibody First   Published

Masson et al. The meiosis-specific   recombinase hDmc1 forms ring structures and interacts with hRad51. EMBO J.   1999 Nov 15;18(22):6552-60. PMID:10562567

Note on publication

This article describes the experiments for   which anti-Rad51 3C10 antibody was created. It was used to visualise Rad51   via Western blot after previous immunoprecipitation with different   antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.