Key features and details | |
Cat. No. | MABL-2779 |
Name | Anti-Pyrrolate Protein mAbs |
Clone No. | AFD- 3C8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody reacts specifically with pyrrolated protein and does not cross react with DNA. Lysine N-pyrrolation is a post translational modification, a unique covalent modification that converts lysine residues to Nε-pyrrole-L-lysine (pyrK). It imparts electronegative properties to proteins, causing them to mimic DNA and the proteins acquire an electrical conductivity via lysine N-pyrrolation. |
Alternative Name | pyrP; Lysine N-pyrrolation; pyrK |
UniProt | |
Immunogen | The original antibody was isolated from the repertoire of the B-1a cell populations in the apoE deficient (apoE−/−) mice. Spleen cells from male apoE−/− mice at 21 weeks of age were fused with P3/U1 murine myeloma cells, and after screening based on specific binding to pyrBSA |
Application Notes | This antibody is recommended for determination of pyrrolated proteins in a sample. The binding reactivity of this antibody towards pyrrolated-BSA protein was determined using ELISA. This antibody does no cross react with DNA in ELISA (PMID: 35031322). |
Antibody First Published | Lim et al. Unique B-1 cells specific for both N-pyrrolated proteins and DNA evolve with apolipoprotein E deficiency. J Biol Chem. 2022 Jan 11;101582. PMID:35031322 |
Note on publication | This paper evaluates the role of pyrrolated proteins in innate immunity using antibodies from apoE-deficient (apoE−/−) hyperlipidemic mice. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
