Key features and details | |
Cat. No. | MABL-2785 |
Name | Anti-Rabbit IgG mAbs |
Clone No. | AFD- A10B |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, WB, ELISA |
Species Reactivity | Rabbit |
Basic Information | |
Specificity | The antibody recognizes the constant heavy chain 1 (CH1) domain of rabbit IgG. |
Alternative Name | immunoglobulin gamma |
UniProt | |
Immunogen | The antibody was originally generated by immunizing BALB/c mice with purified rabbit IgG. |
Application Notes | Different formats of the antibody (scFv-cys, scFv, Fab and IgG) were employed for the construction of biosensors used for antigen detection in complex samples. In scFv-cys, one glycine of the linker was replaced with a cysteine, which determine an orientation of the scFv fragments on the sensor surface. The performance, in terms of sensitivity and selectivity, in biosensors prepared with scFv-cys, scFv, Fab and IgG was compared. The scFv-cys based biosensors displayed greater assay sensitivity and exhibited less nonspecific activity than intact monoclonal IgG and Fab fragment-based immunosensors. The scFv-cys biosensor surface was easily regenerated by stripping the rabbit IgG from the surface using an acidic solution. Moreover, western blot analysis showed purified scFv migrated as a single band at a MW of ~32 000 and did not appear to be degraded and the specificity of IgG1, Fab fragment and scFv towards rabbit IgG was determined by ELISA. (PMID: 15679346) |
Antibody First Published | Shen et al. Single-chain fragment variable antibody piezoimmunosensors Anal Chem. 2005 February 1; 77(3):797–805. PMID:15679346 |
Note on publication | The paper describes a biosensor with high diagnostic potential for rapid and sensitive detection of antigens in complex biological samples. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
