Key features and details | |
Cat. No. | MABL-2780 |
Name | Anti-Quadruplex DNA mAbs |
Clone No. | AFD- 1H6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody binds to quadruplex DNA with a Kd of 0.33 nM. Binds to both types of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2) and Ver-3 (d(TGGGGG(TTAGGG)2T). |
Alternative Name | G4 DNA |
UniProt | |
Immunogen | Oxy-2 or d(TTTTGGGG)2. |
Application Notes | This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats. Ab00389-1.1 was used by Hansel-Hertsch et al. 2016 (PMID: 27618450) in immunofluorescence staining (in comparison to the BG4 anti-G quadruplex antibody, c.f. Ab00174). |
Antibody First Published | Henderson et al. Detection of G-quadruplex DNA in mammalian cells. Nucleid Acids 2014; 42(2):860-869 PMID:24163102 |
Note on publication | Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
