Key features and details | |
Cat. No. | MABL-2710 |
Name | Anti-Podocalyxin mAbs |
Clone No. | AFD- mAb4F10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF |
Species Reactivity | Rabbit, Rat, Human |
Basic Information | |
Specificity | The antibody is specific for human podocalyxin. The epitope recognized by the antibody is located on the intracellular domain of podocalyxin. Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes in the glomerulus (Bowman's capsule) in the kidneys. It is a member of the CD34 family of transmembrane sialomucins. |
Alternative Name | PC; PCLP-1; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; 4F10 |
UniProt | O00592 |
Immunogen | The original antibody was generated by immunizing BALB/C mice with purified human podocalyxin-glutathione S-transferase (GST) fusion protein containing the intracellular, transmembrane, and a part of the extracellular domain of human PCLP. |
Application Notes | The antibody recognized rabbit PCLP1 in human renal cortical sections on immunofluorescence. The antibody detected PCLP by western blot analysis. The antibody recognized and immunoprecipitated rat podocalyxin (Kershaw et al., 1997; PMID: 9188463). The antibody detected PCLP in HEV of tonsil by immunofluorescence analysis (Sassetti et al., 1998; PMID: 9625756). |
Antibody First Published | Kershaw et al. Molecular cloning and characterization of human podocalyxin- like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin. J Biol Chem. 1997 Jun 20;272(25):15708-14. doi: 10.1074/jbc.272.25.15708. PMID:9188463 |
Note on publication | The original paper describes the generation and characterization of the original antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
