Key features and details | |
Cat. No. | MABL-2711 |
Name | Anti-Podoplanin mAbs |
Clone No. | AFD-Pmab-295 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IHC |
Species Reactivity | Elephant |
Basic Information | |
Specificity | This antibody is specific for elephant Podoplanin (elePDPN). PDPN is an essential marker of lung type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. |
Alternative Name | PDPN; elePDPN; Elephant PDPN; Elephant podoplanin |
UniProt | G3T5I4 |
Immunogen | The original antibody was raised by immunizing BALB/c mice four times with three keyhole limpet hemocyanin-conjugated elePDPN peptides, including 38-EGGMVIPGVEDNMV-51, + C-terminal cysteine, followed by a final booster shot. |
Application Notes | This antibody recognized both elePDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells and endogenous elePDPN-expressing LACF-NaNaI cells by flow cytometry (FC) and Western blotting (WB). Kinetic analyses using FC showed that this antibody's KD for CHO/elePDPN was 1.5 × 10-8 M. Furthermore, this antibody detected elePDPN-expressing cells using immunohistochemistry (IHC) (Kudo et al., 2022; PMID: 35917562). An ELISA with the alanine-substituted peptides of the elePDPN extracellular domain (amino acids 38–51) revealed that the binding epitope of this antibody includes Met41, Pro44, and Glu47 of elePDPN (Okada et al., 2022; PMID: 35917553). |
Antibody First Published | Kudo et al. Development of a Monoclonal Antibody PMab-295 Against Elephant Podoplanin Monoclon Antib Immunodiagn Immunother. 2022 Aug;41(4):194-201. doi: 10.1089/mab.2022.0007 PMID:35917562 |
Note on publication | The original publication describes the development and characterization of a novel monoclonal antibody, PMab-295, that specifically recognizes elephant podoplanin (elePDPN). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
