Key features and details | |
Cat. No. | MABL-2709 |
Name | Anti-Podocalyxin mAbs |
Clone No. | AFD- mAb3D3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for human podocalyxin. The epitope recognized by the antibody is located on the extracellular domain of podocalyxin.Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes in the glomerulus (Bowman's capsule) in the kidneys. It is a member of the CD34 family of transmembrane sialomucins. |
Alternative Name | PC; PCLP-1; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; 3D3 |
UniProt | O00592 |
Immunogen | The original antibody was generated by immunizing BALB/C mice with purified human podocalyxin-glutathione S-transferase (GST) fusion protein containing the intracellular, transmembrane, and a part of the extracellular domain of human PCLP. |
Application Notes | The antibody recognized rabbit PCLP1 in human renal cortical sections on immunofluorescence. The antibody detected PCLP by western blot analysis (Kershaw et al., 1997; PMID: 9188463). The antibody detected PCLP in HEV of tonsil by immunofluorescence analysis. The antibody immunoprecipitated PCLP from colon carcinoma cell lysate (Thomas et al., 2009; PMID: 19118161). |
Antibody First Published | Kershaw et al. Molecular cloning and characterization of human podocalyxin- like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin. J Biol Chem. 1997 Jun 20;272(25):15708-14. doi: 10.1074/jbc.272.25.15708. PMID:9188463 |
Note on publication | The original paper describes the generation and characterization of the original antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
