Anti-2.4mAbs

Cat. No.

MABL-301

Name

Anti-2.4mAbs

Clone No.

AFD-S73-2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, ELISA, IF

Species Reactivity

Species independent

Basic Information

Specificity

This antibody is specific for 2.4[2.8]2.4-linked   Kdo tetrasaccharide. Which is a partial structure of chlamydial   lipopolysaccharide (LPS).

Alternative Name

Kdo; alpha-Kdo; 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; 3- deoxy-alpha-D-manno-oct-2-ulopyranosonic   acid; Chlamydia lipopolysaccharide (LPS) component

UniProt

Immunogen

This antibody was raised by  immunizing mice with Kdo(2f8)[Kdo(2f4)]Kdo(2f4)Kdo(2f6)GlcNAc4P-(1f6)GlcNAcol-BSA  and further immunizing them with Kdo(2f8)[Kdo(2f4)]Kdo-BSA.

Application Notes

To screen hybridomas for this   antibody ELISA was performed on neoglycoconjugates and immunofluorescence was   performed on cells infected with either C. trachomatis or C. psittaci.   Surface plasmon resonance was performed on Kdo(2f8)Kdo(2f4)KdoBSA (164, 1650,   and 2460 RUs), Kdo(2f4)Kdo(2f4)Kdo-BSA, Kdo(2f4)Kdo BSA and BSA(Sigma), as a   reference protein using the mouse version of this antibody (Brooks et al,   2010; pmid:20000757).

Antibody First Published

Brooks et al. Antibodies raised   against chlamydial lipopolysaccharide antigens reveal convergence in germline   gene usage and differential epitope recognition. Biochemistry. 2010 Jan   26;49(3):570-81. PMID:20000757

Note on publication

The structures of antigen-binding   fragments from two related monoclonal antibodies have been determined to high   resolution in the presence of several carbohydrate antigens raised against   chlamydial lipopolysaccharide.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.