Key features and details | |
Cat. No. | MABL-304 |
Name | Anti-25-hydroxyvitamin D mAbs |
Clone No. | AFD- AbAb1-VD |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | colloidal gold immunochromatography, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds 25-hydroxyvitamin D. Vitamin D (calciferol) is an essential fat-soluble vitamin responsible for absorption and regulation of calcium and phosphorus, maintaining bone health, regulating immune function, preventing cardiovascular disease, and regulating fat and energy metabolism. It is important for the typical growth and development of bones and teeth and for improving resistance to certain diseases. Deficiency of vitamin may lead to osteoporosis or rickets. |
Alternative Name | 25-OH-VD; 25(OH)D; vitamin D; calciferol |
UniProt | |
Immunogen | The original antibody was generated by subcutaneously immunizing New Zealand rabbits with a conjugate of 25-OH-VD and BSA emulsified with an equal amount of Freund's complete adjuvant. |
Application Notes | This antibody binds 25-hydroxyvitamin D in an ELISA with an EC50 of 9.01 ng/mL and the IgG version of the antibody as a binding affinity in the sub-picomolar range. This antibody in combination with AbAb2-VD can be used in the development of double-antibody sandwich colloidal gold immunochromatography test strips, where this antibody is used to prepare Au-antibody complex and AbAb2-VD is used for T line coating. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
