Key features and details | |
Cat. No. | MABL-299 |
Name | Anti-2.4mAbs |
Clone No. | AFD-S25-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, WB, ELISA, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS). |
Alternative Name | Kdo; alpha-Kdo; 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; 3- deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; Chlamydia lipopolysaccharide (LPS) component |
UniProt | |
Immunogen | This antibody was generated by immunizing a BALB/c mouse with KD03-GlcNAc-BSA. |
Application Notes | An enzyme immunoassay was performed on LPS-BSA complexes and synthetic BSAglycoconjugates using the mouse version of this antibody it showed reactivity to r595-207 LPS and to KD03- GlcNAc-BSA. Furthermore this antibody was used for immunofluorescence on L929 monolayers infected with C.trachomatis serotype L2 or C. psittaci 6BC at a dose resulting in >80% infected cells within 24 to 36 h, the antibody gave a positive result for both infections. Additionally western blots with LPS r595-207 and R595 as the antigen were performed using this antibody, it was postive for r595-207 and negative for R595 (Fu et al, 1992; pmid:1372290). An ELISA was performed on chlamydial LPS fragments using the mouse version of this antibody it bound most strongly to trisaccharides and tetrasaccharides (Evans et al, 2011; pmid:21543444). |
Antibody First Published | Fu et al. A synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart. Infect Immun. 1992 Apr;60(4):1314-21. PMID:1372290 |
Note on publication | The tetrasaccharide 3-deoxy-a-D-manno-2-octulosonic acid (ac-KDO)(2--8)-ce-KDO(2- >4)-ce-KDO(2-->6)- IGIcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial glycoconjugate antigen. Mice were immunized with the glycoconjugate to prepare chlamydiaspecific monoclonal antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
