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Anti-SIRP g mAbs

简要描述:

Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T- cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells..

产品参数:

Cat. No.:MABL-2915

Species:Engineer

Species Reactivity:Human

Type:Recombinate

Application:IP, Block, ELISA, FC

详细介绍

Key features and   details


Cat. No.

MABL-2915

Name

Anti-SIRP g mAbs

Clone No.

AFD- OX-119

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, Block, ELISA, FC

Species Reactivity

Human

Basic Information


Specificity

Hybridoma supernatants were initially   screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to   eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and   SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T   cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion.   Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced   antigen-specific T-cell proliferation and costimulates T-cell activation.   Expressed on CD4+ T- cells, CD8+ T-cells, CD56-bright natural killer (NK)   cells, CD20+ cells, and all activated NK cells..

Alternative Name

CD172g; OX119; MRC OX-119; MRC OX119;   SIRP-g; SIRPg; SIRP gamma; SIRP-gamma; CD172 antigen-like family member B;   signal-regulatory protein beta; CD172-g

UniProt

Q9P1W8

Immunogen

OX-119 was prepared by immunizing BALB/c   mice with a chimeric protein made up of human SIRPg and a leader peptide of   rat CD4 domain (SIRPg-CD4 ).

Application Notes

OX-119 is able to perform staining of   peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis.   OX-119 can also be used in IP, FC and ELISA.

Antibody First   Published

Brooke et al Human lymphocytes interact   directly with CD47 through a novel member of the signal regulatory protein   (SIRP) family. J Immunol 173:2562-70 2004 PMID:15294972

Note on publication

Describes the determintion of OX-119   specificity by ELISA, FC and IP and decribes use of OX-119 to observe the   distribution of SIRPg expression and its ability to block SIRPg-mediated   apoptosis.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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