欢迎来到西安迈博睿(Mabioway)生物科技有限公司 Call: 15339069646 | E-mail: info@mabioway.cn 在线留言 |

服务热线

15339069646
网站导航
当前位置: 首页 > 产品展示 > 抗体产品 > 一抗 > 其他抗体
其他抗体

Anti-Sigma1 mAbs

简要描述:

The structure of 5C6 against Sigma1 was solved crystallographically to 3.7 angstrom resolution (Dietrich et al, 2017). The 5C6 Fab regions each contact the upper part of the Sigma1 ‘head‘, which forms a beta-spiral repeat structure. The VH CDRs contact the antigen more extensively than the VL CDRs, resulting in a discontinuous binding interface. Reoviruses bind to cell-surface glycans as coreceptors, and in particular T1L reovirus is known to bind the glycan GM2 to facilitate infectivity (Reiss et al, 2012). The 5C6 binding site on Sigma1 partially overlaps with its GM2 binding site, blocking viral infectivity by sterically inhibiting receptor binding.

产品参数:

Cat. No.:MABL-2910

Species:Engineer

Species Reactivity:​Reovirus strain T1L

Type:Recombinate

Application:crystallization, IP, NTRL, FC, IF

详细介绍

Key features and   details


Cat. No.

MABL-2910

Name

Anti-Sigma1 mAbs

Clone No.

AFD- 5C6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, IP, NTRL, FC, IF

Species Reactivity

Reovirus strain T1L

Basic Information


Specificity

The structure of 5C6 against Sigma1 was solved crystallographically to 3.7 angstrom resolution (Dietrich et al,   2017). The 5C6 Fab regions each contact the upper part of the Sigma1 'head', which forms a beta-spiral repeat structure. The VH CDRs contact the antigen more extensively than the VL CDRs, resulting in a discontinuous binding   interface. Reoviruses bind to cell-surface glycans as coreceptors, and in   particular T1L reovirus is known to bind the glycan GM2 to facilitate infectivity (Reiss et al, 2012). The 5C6 binding site on Sigma1 partially overlaps with its GM2 binding site, blocking viral infectivity by sterically   inhibiting receptor binding. 

Alternative Name

Outer capsid protein sigma1; sigma1 protein;   Reovirus strain T1L sigma 1; strain T1L S1; T1L S1; type 1 haemagglutinin; type 1 HA; T1L HA

UniProt

P04506

Immunogen

Murine antibodies were produced by immunising   CAF1 mice with reovirus strain T3D, with subsequent immunisation boosts using   UV-inactivated strain T1L reovirus. Immunised mice produced antibodies   recognising both T3D and T1L. Splenocytes were obtained from immunised mice   and fused with the P3Ag8.653 myeloma cell line to generate hybridomas.

Application Notes

5C6 was shown recently to block reoviral   infection of L929 mouse fibroblasts in a dose- dependent manner, using   immunofluorescence staining (Dietrich et al, 2017). Fibroblasts were   incubated with virus-antibody mixtures, and immunofluorescence staining of   the cells enabled detection of infectivity. 5C6 is highly specific for the   T1L reoviral strain, as opposed to the T3D strain which was also tested. 5C6   was also used to immunoprecipitate reoviral particles.

Antibody First   Published

Virgin et al. Monoclonal antibodies to   reovirus reveal structure/function relationships between capsid proteins and   genetics of susceptibility to antibody action. J Virol. 1991   Dec;65(12):6772-81. PMID:1719233

Note on publication

Describes the generation of a panel of   anti-reovirus (strain T3D and T1L) mAbs,including clone 5C6, using mouse   immunisation and hybridoma generation. mAb specificities were probed using   ELISAs and Western blotting.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


服务咨询

留言框

  • 标题:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

上一篇: Anti-Siglec-7 mAbs

下一篇: Anti-Sigma1 mAbs

推荐产品

联系我们

Copyright © 2012-2029 西安迈博睿(Mabioway)生物科技有限公司 版权所有

地址:陕西省西安市浐灞生态区广运潭大道南段4555号长安大学科技园4号楼A单元2层

电话:15339069646 邮箱:info@mabioway.cn

陕ICP备2021006339号