欢迎来到西安迈博睿(Mabioway)生物科技有限公司 Call: 15339069646 | E-mail: info@mabioway.cn 在线留言 |

服务热线

15339069646
网站导航
当前位置: 首页 > 产品展示 > 抗体产品 > 一抗 > 其他抗体
其他抗体

Anti-Sigma1 mAbs

简要描述:

9BG5 was identified as a clone which significantly neutralised the strain T3D reovirus Sigma1 as assayed using viral titre of infection (Burstin et al, 1982). 9BG5 was able to immunoprecipitate strain T3D Sigma1, however some cross-reactivity was observed with strain T1L reoviral Sigma1 (Burstin et al, 1982; Dietrich et al, 2017). Recently, the interaction between 9BG5 and strain T3D reoviral Sigma1 was shown crystallographically (Dietrich et al, 2017). 9BG5 binds to the Sigma1 head region, with 5 CDRs contacting a relatively flat, contiguous epitope.

产品参数:

Cat. No.:MABL-2911

Species:Engineer

Species Reactivity:Reovirus strain T3D

Type:Recombinate

Application:crystallization, IP, RIA, FC

详细介绍

Key features and   details


Cat. No.

MABL-2911

Name

Anti-Sigma1 mAbs

Clone No.

AFD- 9BG5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, IP, RIA, FC

Species Reactivity

Reovirus strain T3D

Basic Information


Specificity

9BG5 was identified as a clone which significantly neutralised the strain T3D reovirus Sigma1 as assayed using   viral titre of infection (Burstin et al, 1982). 9BG5 was able to  immunoprecipitate strain T3D Sigma1, however some cross-reactivity was observed with strain T1L reoviral Sigma1 (Burstin et al, 1982; Dietrich et al, 2017). Recently, the interaction between 9BG5 and strain T3D reoviral   Sigma1 was shown crystallographically (Dietrich et al, 2017). 9BG5 binds to   the Sigma1 head region, with 5 CDRs contacting a relatively flat, contiguous   epitope.

Alternative Name

T3D HA; Outer capsid protein sigma1; sigma1   protein; Reovirus strain T3D sigma 1; strain T3D S1; T3D S1; type 3   haemagglutinin; type 3 HA

UniProt

P03528

Immunogen

Murine antibodies were produced by immunising   BALB/c mice with an injection of strain TD3 reovirus. Splenocytes from   immunised mice were obtained and fused with the NS1 mouse myeloma cell line   to generate a hybridoma.

Application Notes

9BG5 effectively neutralised reovirus strain T3D activity, and showed some inhibition of erythrocyte haemagglutination in virus-erythrocyte suspensions (Burstin et al, 1982). 9BG5 was also used to   immunoprecipitate radiolabelled viral Sigma1 from L929 mouse fibroblast   supernatants. More recently, 9BG5 was shown to cross-react with Sigma1 from   the reovirus strain T1L, and contributed to blocking adherence of both T1L   and T3D virions to CHO cells shown by flow cytometry (Dietrich et al, 2017).

Antibody First   Published

Burstin et al. Evidence for functional   domains on the reovirus type 3 hemagglutinin. Virology. 1982   Feb;117(1):146-55. PMID:6175082

Note on publication

Describes the generation of a panel of   anti-reovirus (strain T3D) mAbs using generated murine hybridomas which   recognised different functional epitopes of the viral Sigma1 protein.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


服务咨询

留言框

  • 标题:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

推荐产品

联系我们

Copyright © 2012-2029 西安迈博睿(Mabioway)生物科技有限公司 版权所有

地址:陕西省西安市浐灞生态区广运潭大道南段4555号长安大学科技园4号楼A单元2层

电话:15339069646 邮箱:info@mabioway.cn

陕ICP备2021006339号