Key features and details | |
Cat. No. | MABL-2762 |
Name | Anti-Protein C mAbs |
Clone No. | AFD- IgG1-7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, Purification, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specifically recognizes and binds the ProC epitope tag comprising the amino acid sequence 'EDQVDPRLIDGK'. This antibody binds the zymogen form of human Protein C in the activation peptide (spanning the thrombin cleavage site) and blocks protein C activation by thrombin–thrombomodulin both in vitro and in vivo. It does not react with activated protein C. |
Alternative Name | PROC; Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; HPC-4; HPC4; Fab-7 |
UniProt | P04070 |
Immunogen | The original antibody was generated from the original mouse antibody HPC-4 by performing site directed mutagenesis, consensus and resurfacing methods. |
Application Notes | This antibody is the humanized version of anti-Protein C antibody HPC-4. The binding of this antibody to protein C is calcium dependent. This humanized anti-protein C antibody exhibited calcium dependent binding which was significantly increased over that of the murine antibody HPC-4. The binding characterization of this antibody was performed using ELISA (PMID: 9680194). This antibody can be used to purify protein tagged with this 'EDQVDPRLIDGK' epitope. |
Antibody First Published | O'Connor et al. Humanization of an antibody against human protein C and calcium-dependence involving framework residues. Protein Eng. 1998 Apr;11(4):321-8. PMID:9680194 |
Note on publication | Describes the generation of humanized version of anti-Protein C antibody HPC-4. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
