Key features and details | |
Cat. No. | MABL-2763 |
Name | Anti-prothrombin mAbs |
Clone No. | AFD- P11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The original antibody is specific for prothrombin. It cross-reacts with beta 2-glycoprotein-1. |
Alternative Name | EC:3.4.21.5; Coagulation factor II |
UniProt | P00734 |
Immunogen | A phage display library was generated from a patient with anti-Ox-LDL antibody, and the antibody was isolated by panning against prothrombin. |
Application Notes | The specificity of the original format of the antibody was confirmed by competitive ELISA. The original format of the antibody reacted with beta 2-glycoprotein-1 and prothrombin and showed comparable binding affinity to both antigens, with Kd values of 1.6 uM for beta 2-glycoprotein-1 and 3.2 uM for prothrombin. |
Antibody First Published | Chukwuocha et al., Isolation, characterization and sequence analysis of five IgG monoclonal anti-beta 2-glycoprotein-1 and anti-prothrombin antigen-binding fragments generated by phage display. J Immunol. 1999 Oct 15;163(8):4604-11. PMID:10510405 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
