Key features and details | |
Cat. No. | MABL-2729 |
Name | Anti-POVPC/PGPC mAbs |
Clone No. | AFD- 10C12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, SPR, ELISA, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for oxidized phospholipids POVPC and PGPC. These are lipids play an important role in cardiovascular disease. |
Alternative Name | POVPC; PGPC |
UniProt | |
Immunogen | The mouse version of this antibody was generated by feeding an ApoE KO mice with a high- fat western diet. |
Application Notes | When injected in ApoE KO mice, the mouse version of this antibody decreased accumulation of macrophages in the brachiocephalic artery and decreased OxPL-induced inflammatory response (Cherepanova and Owens, 2014). To study the binding of its ligands a surface plasmon resonance experiment was preformed on POVPC, PGPC and DMPC using the mouse version of this antibody. Modified Russell-Movat (Movat) staining for morphometric analysis of the brachiocephalic arteries was preformed using the mouse version of this antibody. To confirm the binding of this antibody to POVPC and PGPC an ELISA was preformed (Cherepanova et al, 2019; pmid:31645128). |
Antibody First Published | Cherepanova et al. Novel Autoimmune IgM Antibody Attenuates Atherosclerosis in IgM Deficient Low-Fat Diet-Fed, but Not Western Diet-Fed Apoe-/- Mice Arterioscler Thromb Vasc Biol. 2020 Jan;40(1):206-219. PMID:31645128 |
Note on publication | A novel IgM autoantibody was generated, 10C12, from the spleens of Apoe-/- mice fed a long-term Western diet, that demonstrated potent OxPL neutralizing activity in vitro and the ability to inhibit macrophage accumulation within arteries of Apoe-/- mice fed a Western diet for 4 weeks. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
