Key features and details | |
Cat. No. | MABL-2769 |
Name | Anti-PSMA mAbs |
Clone No. | AFD- 7F12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds a conformational epitope on the human PSMA. This antibody can bind native PSMA protein but were unreactive with any fusion protein containing a portion of the PSMA sequence. Heat denaturation of PSMA also results in loss of binding. |
Alternative Name | PSM; FOLH1; FOLH; FGCP; GCPII; mGCP; NAALAD1; GIG27; Prostate specific membrane antigen; Glutamate carboxypeptidase 2; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; Pteroylpoly-gamma-glutamate carboxypeptidase |
UniProt | Q04609 |
Immunogen | The original antibody was generated by immunizing mice with cell membranes and purified PSMA protein isolated from LNCaP cells. |
Application Notes | This antibody was capable of detecting membrane fractions from human prostatic adenocarcinoma LNCaP and PC3 cells in a solid phase ELISA. This antibody was used for immunoprecipitation of PSMA from LNCaP cell detergent lysates. Antibody binding competition assays were done using flow cytometry and it revealed that 7F12 binds an epitope closed to another murine anti-PSMA antibody 3C6. This antibody was shown to mediated antibody dependent cellular cytotoxicity in human prostatic adenocarcinoma LNCaP cell targets with PBMC's from two donors (WO2003064606). This antibody was also used in the treatment of in vivo tumor xenografts with a combination of Taxotere® (US20090297438). |
Antibody First Published | Deo et al. Human monoclonal antibodies to prostate specific membrane antigen (PSMA). WO2003064606 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
