Key features and details | |
Cat. No. | MABL-2715 |
Name | Anti-Polyglutamine mAbs |
Clone No. | AFD- MW1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the extended polyglutamine repeats (GQQQQQQQQQQG) of the mutant huntingtin exon 1. It does not bind the wild type Htt. |
Alternative Name | PolyQ; HTT; htt; Huntington disease protein; HD protein; huntingtin; Glutamine repeats; Atrophin-1 |
UniProt | P42858 |
Immunogen | This antibody was raised against a 53 amino acid fragment of atrophin-1 containing poly Q (19) and purified from ascites fluid by protein G affinity chromatography. |
Application Notes | This antibody strongly prefers to bind the expanded polyQ repeat forms of the mutant huntingtin and displayed no detectable binding to normal huntingtin in Western blot assay. These results suggested that the polyQ domain can assume different conformations that are distinquishable by mAbs. Immunohistochemistry with wild type (WT) and mutant Htt transgenic mouse (R6) brains shows that different antibodies have different staining patterns. MW1 shows punctate cytoplasmic and neuropil staining in immunohistochemical staining (PMID: 11719267). When MW1 scFv is co-transfected in 293 cells with htt exon 1 containing an expanded polyQ domain, it stimulate htt aggregation and apoptosis (PMID: 11792860). |
Antibody First Published | Ko et al. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Research Bulletin (2001); Volume 56; Issues 3–4; Pages 319-329 PMID:11719267 |
Note on publication | Describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
