Key features and details | |
Cat. No. | MABL-2742 |
Name | Anti-Prion protein mAbs |
Clone No. | AFD- mab 132 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, WB, ELISA, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody recognizes residues 119–127 of mouse PrP and specifically detects PrPSc under partially denatured conditions. |
Alternative Name | PrPC; ASCR; PrP27-30; PrP33-35C; CD230 |
UniProt | P04156 |
Immunogen | The original antibody was generated by immunizing PrP gene-ablated mice with PrP. |
Application Notes | The antibody was employed for detection of PrP in N2a-3 cells, ScN2a-3-22L cells, N2a- 3 cells infected with the Chandler strain (ScN2a-3-Ch) and in GT1-7 cells persistently infected with the 22L strain (ScGT1-7-22L) in immunofluorescence analysis. Fluorescent signals in uninfected cells remained at a background level, while granular signal were detected in infected cells (Yamasaki et al., 2012; PMID: 22090211). The specificity of the original format of the antibody was confirmed by ELISA analysis (Kim et al., 2004; PMID: 15003861). The antibody could distinguish between prion-infected cells (ScN2a-3-22L) from uninfected cells (N2a-3) in a cell-based ELISA. The cell-based ELISA was successful also with ScN2a-3-Ch and ScGT1-7-22L. (Shan et al., 2016; PMID: 27565564). The antibody reacted with denatured PrPC and PrPSc in western blot analysis. The antibody reacted with mouse ovine and bovine PrP Sc in western blot analysis (Kim et al., 2004; PMID: 15003861). |
Antibody First Published | Kim et al. Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies Virology. 2004 Mar 1;320(1):40-51. PMID:15003861 |
Note on publication | The paper describes the generation of a panel of antibodies against prion protein. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
