Key features and details | |
Cat. No. | MABL-2714 |
Name | Anti-Polyglutamine mAbs |
Clone No. | AFD- 1C2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody bind the extended polyglutamine repeats found in the TATA binding protein. It is reported that the affinity of this mAb is proportional to the length of the polyglutamine chain and that this antibody recognizes polyQ stretches with 11, 22 and 41 amino acids. |
Alternative Name | PolyQ; Glutamine repeats; TATA binding protein; TBP |
UniProt | P20226 |
Immunogen | This antibody was raised against the full length TATA binding protein (TBP) containing a poly Q sequnce with 38 repeats of glutamine and collected from the ascites fluid of the mice. |
Application Notes | This antibody recognizes recombinant and endogenous HeLa cell TBP in Western blots. Epitope mapping of the antibody was done using ELISA by incubating the mAbs with synthetic overlapping peptides having 150 amino acids from the N-terminal of the HeLa cell TBP. Thia antibody immunoprecipitates natibe TFIID from phosphocellulose 1 M KCl fractions indicating that the N-terminal region of TBP is not blocked by TBP binding proteins. It is reported that mAb 1C2 selectively inhibited in vitro transcription from TATA-containing, but not from TATA-less promoters, irrespective of whether they were transcribed by Pol II or Pol III (PMID:7510635). This antibody is reported to successfully recognize the polyglutamine chains of mutated alleles of protein from several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntington's disease and spinocerebellar ataxia type 2,3 and 7 (PMID: 7477379). |
Antibody First Published | Lescure et al. The N-terminal domain of the human TATA-binding protein plays a role in transcription from TATA-containing RNA polymerase II and III promoters. EMBO Journal (1994); Vol. 13 ;no.5 pp. 1; 166 - 1175. PMID:7510635 |
Note on publication | Describes the characterization and role of this antibody to inhibit in-vitro transcription from TATA-containing promoter. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
