Key features and details | |
Cat. No. | MABL-2716 |
Name | Anti-PorA protein mAbs |
Clone No. | AFD- 207,B-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Neisseria meningitidis |
Basic Information | |
Specificity | This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence 'ASGQ'. The binding of this antibody was enhanced by the addition of a C-terminal valine residue (ASGQv). |
Alternative Name | PIA; Protein IA; Class 1 protein; P1.7; P1.7 serosubtype; Major outer membrane protein P.IA |
UniProt | E6MXW0 |
Immunogen | The original antibody was generated by immunizing mice with outer membrane vesicles from Neisseria meningitidis strain 188/87 (B:15:P1.7,16d ET-5). |
Application Notes | This antibody was reported to have bactericidal and opsonophagocytic effects for P1.7- expressing meningococci. This antibody demonstrated high opsonophagocytic activity with >80% of polymorphonuclear leukocytes (PMNs) exhibiting measurable respiratory burst (RB) at a concentration of the MAbs of 10 μg/ml. The phagocytic activity was tested against log-phase-growth strain 44/76-SL bacteria by using human serum as a complement source, human PMNs as effector cells, and increasing concentrations of the antibody as the opsonin. The test was performed by using flow cytometry to measure the respiratory burst induced in the effector cells following phagocytosis of the test strain. The epitope mapping of this antibody was done using ELISA (PMID: 10722576). |
Antibody First Published | Wang et al. Functional Activities and Immunoglobulin Variable Regions of Human and Murine Monoclonal Antibodies Specific for the P1.7 PorA Protein Loop of Neisseria meningitidis. Infect Immun. 2000 Apr; 68(4): 1871–1878. PMID:10722576 |
Note on publication | The paper aims to compare the functional and molecular characteristics of a human and three mouse antibodies specific for the PorA P1.7 serosubtype. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
