Anti-N-acyl homoserine lactones mAbs

Cat. No.

MABL-2452

Name

Anti-N-acyl homoserine lactones mAbs

Clone No.

AFD- RS2-1G9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, infectious disease, ELISA

Species Reactivity

P. aeruginosa

Basic Information

Specificity

Binds 3-oxo-C12-HSL with a Kd of 150 nM

Alternative Name

HSL;AHL

UniProt

Immunogen

The 4-methoxyphenyl amide AHL analog.

Application Notes

This antibody, originally raised in mice,   binds to several homoserine lactones from P. aeruginosa. Homoserine lactones   (HSL) are signalling molecules in bacteria used for quorum sensing, required   for successful estabilshment of infecion. RS2-1G9 interferes with quorum   sensing and was shown to protect murine bone marrow derived macrophages, from   the cytotoxic P. aeruginosa quorum sensing signalling molecule   N-3-oxo-dodecanoyl-homoserine lactone (PMID: 18304641).

Antibody First   Published

Kaufmann et al. 2006 Antibody Interference   with N-Acyl Hosoerine Lacone- Mediated Bacterial Quorum Sensing. Journal of   the American Chemical Society. 2006; 128 (9): 2802-2803 PMID:16506750

Note on publication

Describes the design of HSL-analogous   haptens used for the immunization of mice for hybridoma production of the 1G9  antibody as well as charcterization of the inhibibition of quorum sensing by   the produced antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.