Key features and details | |
Cat. No. | MABL-2453 |
Name | Anti-N-cadherin mAbs |
Clone No. | AFD- 8C11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IF, IHC |
Species Reactivity | Hamster, Rabbit, Human |
Basic Information | |
Specificity | This antibody recognizes human, rabbit and hamster extracellular domain of N-cadherin. It does not cross react with this protein in mouse, rat cow and pig. N-cadherin is a calcium-dependent cell adhesion protein. |
Alternative Name | CD325; Cadherin-2; CDw325; Neural cadherin; n-cadherin; ncadherin |
UniProt | P19022 |
Immunogen | This antibody was raised by immunizing mice with an extracellular domain of the recombinant human N-cadherin. |
Application Notes | 8C11 antibody inhibits cells' motility, as it blocks extracellular domain of N-cadherin which is responsible for this function (Kim et al., 2000). This antibody has also been widely used in the detection and analysis of N-cadherin. For instance, it was utilized to identify this protein in A549 cells via immunofluorescence (Shintani et al., 2011). Another group used successfully 8C11 to analyze the levels of N-cadherin expression via Western blot (Chen et al., 2015). Finally, this antibody was used to label N- cadherin in tumor tissues of a metastasis model via immunohistochemistry (Li et al., 2019). |
Antibody First Published | Kim et al. N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility. J Cell Biol. 2000 Dec 11;151(6):1193-206. PMID:11121435 |
Note on publication | This article describes the generation and characterization of the antibody 8C11. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
