Key features and details | |
Cat. No. | MABL-2540 |
Name | Anti-Oestrone-3-glucuronide mAbs |
Clone No. | AFD-4155 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds to E3G with an affinity of 10 nM. |
Alternative Name | E3G |
UniProt | |
Immunogen | Human E3G. |
Application Notes | The antibody is specific for Oestrone-3-glucuronide (E3G), a urinary metabolite of 17β- estradiol, a hormone secreted by the developing ovarian follicles. The monoclonal antibodies can be used clinically to detect levels of E3G in urine samples of women, providing a non-invasive test for determining the fertile period, indicating the time of ovulation. The antibody also has a lower affinity (1 nM) for estriol 3- (β-D-glucuronide), which differs to E3G by a single hydroxyl group. |
Antibody First Published | Trinh CH, Hemmington SD, Verhoeyen ME, Phillips SE. Antibody fragment Fv4155 bound to two closely related steroid hormones: the structural basis of fine specificity. Structure. 1997 Jul 15;5(7):937-48. PMID:9261086 |
Note on publication | Describes the production the monoclonal antibody 4155, which can be clinically useful in the detection of steroid glucuronides concentrations in samples of early morning urine, indicating the fertile period in the females menstrual cycle. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
