Anti-N-acetyl-ganglioside (GM2) mAbs

Cat. No.

MABL-2451

Name

Anti-N-acetyl-ganglioside (GM2) mAbs

Clone No.

AFD- E1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

Gangliosides are glycosphingolipids   expressed on the cell surface membranes of most mammalian cell types. In   ELISA experiments E1 showed a highly-restricted binding specificity,   detecting only GM2 (not GM1 or GM3). The antibody recognises only the N-acetylneuraminic   acid deratives of gangliosides; no N-glycolylneuraminic acid derivatives were   recognised.

Alternative Name

UniProt

Immunogen

The antibody was generated by injecting BALB/c   mice with GM2 ganglioside incorporated into liposomes.

Application Notes

ELISA demonstrated E1 has a   highly-restricted binding specificity, binding only to GM2. IHC studies   showed positivity with E1 in primary lung carcinomas, but no reactivity with   corresponding normal tissues (Alfonso et al, 1995).

Antibody First   Published

Alfonso et al, 1995. T Cell-Independent Cell   Response to Self- Monosialogangliosides: Primary Response Monoclonal   Antibodies Hybridoma. 1995 Jun;14(3):209-16 PMID:7590782

Note on publication

Describes the original generation of E1 and   its characterisation through ELISA and IHC.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.