Key features and details | |
Cat. No. | MABL-1568 |
Name | Anti-Fc gamma receptor III mAbs |
Clone No. | AFD- 3G8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, IP, SPR, Block, FC, IHC |
Species Reactivity | Baboon, Capuchin Monkey, chimpanzee, Cotton-topped Tamarin, Cynomolgus, Marmoset, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey, Human |
Basic Information | |
Specificity | 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity). |
Alternative Name | CD16; low affinity IgG receptor III; FcγRIII; low affinity FcγRIII |
UniProt | Q9ULV2 |
Immunogen | 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells. |
Application Notes | 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies. |
Antibody First Published | Bruenke et al. A recombinant bispecific single-chain Fv antibody against HLA class II and FcgammaRIII (CD16) triggers effective lysis of lymphoma cells. Br J Haematol. 2004 Apr;125(2):167-79. PMID:15059139 |
Note on publication | Describes the construction and characterisation of recombinant bsscFv capable of targetting CD16+ immune cells to maligant B cells resulting in ADCC. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
