Key features and details | |
Cat. No. | MABL-1567 |
Name | Anti-Fc mAbs |
Clone No. | AFD- TP897 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | dot blot assay, ICC, IP, WB, IF |
Species Reactivity | Guinea Pig, Human (weak), Rabbit |
Basic Information | |
Specificity | This antibody recognises Fc fragment of the rabbit IgG. |
Alternative Name | fragment crystallizable region; Fc region |
UniProt | |
Immunogen | This antibody was raised by immunising alpacas with 1.0 mg polyclonal rabbit IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed. |
Application Notes | Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody conjugated with a maleimide- activated HRP was used to detect rabbit IgG via ECL Western blot and it considerably outperformed a polyclonal secondary antibody–HRP conjugate (Pleiner et al., 2018). Similarly, when conjugated with the infrared fluorophore IRDye 800, it was very efficient in detecting nucleoporins (Nups) in a Xenopus laevis egg extract via Western blot (Pleiner et al., 2018). TP897 antibody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated polyclonal rabbit IgG from serum and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018). This nanobody is also recommended for the immunofluorescence detection of both monoclonal and polyclonal primary rabbit antibodies (Pleiner et al., 2018). |
Antibody First Published | Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID: 29263082 |
Note on publication | The article describes the generation and characterisation of this nanobody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
