Anti-Factor H binding protein mAbs

Cat. No.

MABL-1546

Name

Anti-Factor H binding protein mAbs

Clone No.

AFD- JAR41

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Neisseria meningitidis

Basic Information

Specificity

This antibody binds an epitope located on a   conserved region of N-terminal domain of N. meningitidis FHbp, a virulence  factor critical for surviving of the bacteria in the human host. This   antibody binds equally well to fHbp variants, including representative   variants in variant groups 1, 2 or 3. FHbp protein is a bacterial surface   lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis.

Alternative Name

fHbp; GNA1870; Genome-derived Neisseria   antigen 1870; Lipoprotein 2086; JAR 41

UniProt

E6MV22

Immunogen

The original antibody was generated from a human   factor H (fH) transgenic BALB/c mouse immunized with a recombinant fHbp ID 1   (variant group 1).

Application Notes

This antibody broadly   cross-reacts with fHbp sequence variants from all N. meningitidis variant   groups. In combination with second non-bactericidal anti-fHbp mAb like JAR 5,   JAR 41 elicited complement-mediated bactericidal activity in vitro, and   augmented passive protection against meningococcal bacteremia in human fH   transgenic rats (PMID: 22461972). JAR 41 shows concentration- dependent   binding to fHbp ID 1 (variant group 1), fHbp ID 77 (variant group 2) and fHbp   ID 28 (variant group 3) in an ELISA. This antibody specifically binds fHbp   and does not cross react with other meningococcal surface proteins like NadA   and GNA1030. The ability of this antibody to bind 21 fHbp sequence variants   was also measured by ELISA. 

Antibody First   Published

Vu DM, et al. A broadly cross-reactive monoclonal antibody against an epitope on the n-terminus of meningococcal fHbp. Sci Rep. 2012; 2: 341. PMID:22461972

Note on publication

Describes the generation   and characterization of a broadly cross reactive antibody towards fHbp of N.   meningitidis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity  purified

Buffer

PBS with 0.02% Proclin  300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3  months. For longer storage, aliquot and store at - 20⁰C.