Key features and details | |
Cat. No. | MABL-1545 |
Name | Anti-Factor H binding protein mAbs |
Clone No. | AFD- clone4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Neisseria meningitidis |
Basic Information | |
Specificity | This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis. |
Alternative Name | fHbp; GNA1870; Genome-derived Neisseria antigen 1870; Lipoprotein 2086 |
UniProt | E6MV22 |
Immunogen | The recombinant fHbp protein of Neisseria meningitidis was used as the antigen for the immunization of BALB/c mice. Anti-fHbp VH/k chain ribosome display libraries was generated and the actual antibody was obtained by panning the scFv library against the recombinant fHbp protein by using a single round of the ribosome display method via a rabbit reticulocyte lysate system. |
Application Notes | This antibody exhibited high affinity and specificity in the enzyme-linked immunosorbent assay (ELISA) and the whole bacterial cell enzyme-linked immunosorbent assay (Bact- ELISA). The binding affinity of the scFv version of this antibody for fHbp was reported to be Kd= 7.03 nM (PMID: 29737965). |
Antibody First Published | Yarian et al. Identification and characterization of a novel single-chain variable fragment (scFv) antibody against Neisseria meningitidis factor H-binding protein (fHbp) J Med Microbiol. 2018 Jun;67(6):820-827. PMID:29737965 |
Note on publication | Describes the isolation and characterization of an scFv antibody against fHbp protein of N meningitidis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
