Key features and details | |
Cat. No. | MABL-1529 |
Name | Anti-Estrogen receptor mAbs |
Clone No. | AFD- S72 (scFv7) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the amino acid residues 284-310 of human estrogen receptor (hER- α36). The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. |
Alternative Name | ER; ERα36; hER-α36; ESR1; ER-alpha; NR3A1; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1 |
UniProt | P03372 |
Immunogen | This antibody was generated from a phage display library constructed from normal human lymph cells using scFv expression vectors. The scFv antibody was isolated by panning the library against a target peptide comprising amino acid residues 284-310 of human estrogen receptor (hER-α36). |
Application Notes | To characterize the binding of this antibody an ELISA was performed against biotinylated ERα36 using the scFv version. The scFv version of this antibody can also recognize human ERα36 in a western blot assay. And to identify the therapeutic advantages of this antibody, BCAP-37 tumors were implanted in balb/c nude mice after which the scFv version of this antibody was administered. The tumor sizes showed a significant difference with the control group (US20110311517). |
Antibody First Published | Antibodies and methods for treating estrogen receptor-associated diseases. US20110311517 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
