Key features and details | |
Cat. No. | MABL-1530 |
Name | Anti-estrogen receptor alpha mAbs |
Clone No. | AFD- D547 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | EIA, functional assays, IP, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c- Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. |
Alternative Name | Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1 |
UniProt | P03372 |
Immunogen | This antibody was raised by immunizing a Lewis rat, with purified estradiol-receptor complex of calf uterine nuclei |
Application Notes | crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). An EIA was preformed on cytosol from breast cancer tumors using this antibody (Cren et al, 1991; pmid:1911441). COS-7 cells cotransfected with expression plasmids encoding ERa and HA-tagged ERb were immunoprecipitated with this antibody (Ogawa et al, 1998; pmid:9473491). Sucrose Density Gradient Analysis was preformed on GH3 cells (Tate et al, 1984; pmid:6708522). Immunocytochemistry was preformed on the reproductive tract of Female cynomolgus macaques using this antibody (McClellan et al, 1984; pmid:6373239). |
Antibody First Published | Greene et al. Monoclonal antibodies to estrophilin: Probes for the study of estrogen receptors Proc Natl Acad Sci U S A. 1980 Jan;77(1):157-61. PMID:6928610 |
Note on publication | The paper describes the antibody being raised by immunizing lewis rats with purified estradiol-receptor complex of calf uterine nuclei. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
