Key features and details | |
Cat. No. | MABL-1528 |
Name | Anti-Estrogen receptor mAbs |
Clone No. | AFD- JS34/32 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, IP, IHC |
Species Reactivity | Bovine, Rat, Human |
Basic Information | |
Specificity | This antibody is specific for human, bovine and rat estrogen receptor. The different molecular forms of the estrogen receptor also showed no difference in binding affinity.The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. |
Alternative Name | ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1 |
UniProt | P49884 |
Immunogen | This antibody was raised by immunizing Balb/c mice with bovine estrogen receptor purified from calf uterus. |
Application Notes | Aliquots of 16a- [125I]iodo17P-estradiol-labeled receptor were immunoprecipitated with the mouse IgG2b version of this antibody (Moncharmont et al, 1982; pmid:7159574). To assess the localization of estrogen receptor (ER) in rat brain, pituitary and uterus, immunohistochemistry was performed on the brain, pituitary and uterus of rats (Sar and Parikh, 1986; pmid:2422450). To test whether the role of estrogen in breast cancer is dependent on a modified expression of their receptor, immunohistochemistry was performed on samples of human breast cancer using the mouse version of this antibody (Fabris et al, 1987; pmid:3695478). Determination of antibody interaction with microsomal and cytosolic estrogen-binding proteins was carried out by monitoring the shift in sedimentation coefficient of estrogen-estrophile complexes that occurs when they are bound by the mouse version of this antibody. |
Antibody First Published | Moncharmont et al. Monoclonal antibodies against estrogen receptor: interaction with different molecular forms and functions of the receptor. Biochemistry. 1982 Dec 21;21(26):6916-21. PMID:7159574 |
Note on publication | Hybridoma cells have been produced by fusing SP2/O-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a purified preparation of estrogen receptor from calf uterus. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
