Key features and details | |
Cat. No. | MABL-1514 |
Name | Anti-Epiregulin mAbs |
Clone No. | AFD-9E 5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, in vitro, inhibition, SPR, WB, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds the EGF-like domain of human epiregulin, but does not recognize other epidermal growth factor family members. Epiregulin is a ligand of the epidermal growth factor family that upon binding to its epidermal growth factor receptor stimulates proliferative signaling, especially in colon cancer cells. |
Alternative Name | Proepiregulin; EPR; EREG |
UniProt | O14944 |
Immunogen | |
Application Notes | The antibody (IgG) and two humanized versions were used to examine the cell surface and subcellular localization of epiregulin by immunofluorescence staining. The antibody-binding assays were performed using colorectal carcinoma cells (DLD-1, HCT116). The original antibody and the humanized version HM1 were detected predominantly as intense green florescence spots in the cell surface with some faint fluorescence demonstrating subcellular distribution in colon cancer cells. The humanized version HM0 also exhibited weak and diffuse cell surface stained. The cell surface binding efficiency of each antibody to epiregulin expressing cells was determined by flow cytometry on DLD-1 and HCT116 cells. The results of flow cytometry indicated that HM1 bound more efficiently to DLD-1 and HCT116 cells than the same concentration of HM0. Further, HM1 was able to bind epiregulin expressing cells at a relatively low concentration (0.1 ug/mL). Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between the antibody and the humanized versions of the antibody and epiregulin. |
Antibody First Published | Hun Lee et al. Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochem Biophys Res Commun. 2013 Nov 29;441(4):1011-7.PMID:24239549 |
Note on publication | The paper describes the characterization of the original antibody and the construction and expression of two humanized versions with high-affinity targeted cytotoxicity and decreased immunogenicity through resurfacing the variable region and recombination. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
