Anti-Ephrin-B2 mAbs

Cat. No.

MABL-1513

Name

Anti-Ephrin-B2 mAbs

Clone No.

AFD- B11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, inhibition, SPR, WB, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human Ephrin-B2.

Alternative Name

EFNB2; EPLG5; HTKL; LERK5; HTK-L; LERK-5;   HTK ligand; EPH-related receptor tyrosine kinase ligand 5

UniProt

P52799

Immunogen

The original antibody was raised by panning a human antibody against ephrin B2.

Application Notes

The original antibody was used for an ELISA on ephrin B2. This antibody was used for flow cytometry on specific antisera of each member of the ephrinB family (Abéngozar et al., 2012; PMID:22446484), and on human CD138+ cells (Sasine et al., 2024; PMID:38231476). This antibody was used for a western blot on different proteins from ephrinB2 family, it  showed it was only able to bind to ephrinB2. This antibody was used for surface plasmon resonance to determine its binding strength. This antibody was used for a transwell migration assay and a wound healing assay, it showed that the percentage of HUVEC migrated cells was reduced from 80% (untreated) to 50% and 40%, respectively. This antibody was used for a matrigel assay on   HUVEC cells, here it inhibited the formation of tube-like structures by >40%. This antibody was used in vivo on nude or SCID mice with xenografted tumors (BxPC3, SW620, H460), it showed strong tumor inhibition for all these  tumor types (Abéngozar et al., 2012; PMID:22446484). 

Antibody First   Published

Abéngozar et al. Blocking ephrinB2 with   highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and   tumor growth Blood. 2012 May 10;119(19):4565-76. PMID:22446484

Note on publication

In the original paper to investigate the   functional consequences of blocking ephrinB2 activity, 2 highly specific   human single-chain Fv (scFv) Ab fragments against ephrinB2 were generated and   characterized.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.