Key features and details | |
Cat. No. | MABL-1515 |
Name | Anti-Equine IFN gamma mAbs |
Clone No. | AFD-M14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA, FC, IF |
Species Reactivity | Horse |
Basic Information | |
Specificity | This antibody is specific for equine IFN gamma. |
Alternative Name | IFN-g; IFN-gamma; interferon gamma; IFNG |
UniProt | P42160 |
Immunogen | This antibody was raised by immunising mice with recombinant equine IFN-gamma, expressed as a GST fusion protein, produced in E. coli. |
Application Notes | The specificity of this antibody for equine IFN-gamma has been confirmed in in ELISA and Western blot analysis using recombinant equine IFN-gamma derived from E. coli (Gutmann et al, 2005). This antibody has been used in Western blot and immunofluorescence analysis to detect equine IFN-gamma in mammalian cells transfected with IFN-gamma and insect cells infected with equine IFN-gamma-recombinant baculovirus (Gutmann et al, 2005). |
Antibody First Published | Gutmann et al. Characterisation and quantification of equine interferon gamma Vet Immunol Immunopathol. 2005 Mar 10;104(1-2):105-15. PMID:15661336 |
Note on publication | Describes the original generation of this antibody, and its characterisation. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |