Key features and details | |
Cat. No. | MABL-297 |
Name | Anti-2,3-dinor-6-keto-prostaglandin F1α mAbs |
Clone No. | AFD-mAb6kPGF |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds to to 2,3-Dinor-6-keto-prostaglandin F1α with an IC50 of 2.2 pmol and an affinity (Kd) of 2.22 nM. |
Alternative Name | 2,3-dinor-6-keto-prostaglandin F1-alpha |
UniProt | |
Immunogen | Human 2,3-dinor-6-keto PGF1α. |
Application Notes | The antibody binds specifically to 2,3-Dinor-6-keto-prostaglandin F1α, a major urinary metabolite of PGI2 (prostacyclin). PG12 is a product of cyclooxygenase, and acts as a potent vasodilator and inhibitor of platelet aggregation. 2,3-Dinor-6-keto-prostaglandin F1α is a stable urinary metabolite of PGI2, and its levels in the urine can be measured using monoclonal antibodies in immunoassays, giving an indication of the levels of PGI2 produced in the body. |
Antibody First Published | Hiramatsu M, Hayashi Y, Yamamoto S, Hayashi A, Sawada M, Hamanaka N, Yamashita K. Application of an alpha-side chain length-specific monoclonal antibody to immunoaffinity purification and enzyme immunoassay of 2,3-dinor-6-keto-prostaglandin F1α from human urine. Prostaglandins Leukot Essent Fatty Acids. 1994 Feb;50(2):69-79. PMID:8171070 |
Note on publication | Describes the preparation of a monoclonal antibody by immunising a mouse with 2,3-dinor-6-keto-prostaglandin F1α conjugated to bovine serum albumin, and its use for the detection of PG12 in human urine. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
