Key features and details | |
Cat. No. | MABL-294 |
Name | Anti-17ß estradiol mAbs |
Clone No. | AFD-10G6D6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, RIA, WB, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds specifically to 17β estradiol, a mammalian estrogenic steroid hormone that is produced in the ovaries, placenta, and testis. |
Alternative Name | 17-beta-estradiol; E2; ethynyl estradiol; oestradiol |
UniProt | |
Immunogen | estradiol-6-ethyl methoxy carbonyl (EMC)–bovine serum albumin (BSA). |
Application Notes | This antibody binds 17ß estradiol with a sensitivity of 33 ± 9 pg/ml. In women, 17β estradiol plays an important role in the development of secondary sexual characteristics and the maturation of long bones. It is also responsible for the activation of certain oncogenes and can be involved in the development of breast cancer and cancer of the uterine lining. Anti-estradiol antibodies can be useful in the detection of hormone concentrations in serum samples. This can be used therapeutically to monitor follicular maturation in cases of female infertility and estradiol-secreting tumors. |
Antibody First Published | Bettsworth F, Monnet C, Watelet B, Battail-Poirot N, Gilquin B, Jolivet M, Menez A, Arnaud M, Ducancel F. Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments. J Mol Recognit. 2001 Mar-Apr;14(2):99-109. PMID:11301480 |
Note on publication | Describes the role of monoclonal antibodies in measuring concentrations of steroid hormones in human serum samples, and the characterization of two anti-estradiol monoclonal antibodies for this use. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
