Key features and details | |
Cat. No. | MABL-291 |
Name | Anti-17-Hydroxyprogesterone mAbs |
Clone No. | AFD-4B2 (4B2.2.1) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds 17α-hydroxyprogesterone (17-OHP). |
Alternative Name | 17-OH; 17-OHP; 17α-hydroxyprogesterone |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with 17-OHP-bovine serum albumin conjugate as an immunogen. |
Application Notes | This antibody has good affinity for 17-OHP, a chemical intermediate involved in the biosynthesis of many endogenous steroids like androgens, estrogens, glucocorticoids, and mineralocorticoids, as well as neurosteroids. Congenital adrenal hyperplasia (CAH) is an autosomal recessive disorder caused by the deficiency of one of the five enzymes involved in the biosynthesis of corticosteroids. The most common form of the disease is the deficiency of 21-hydroxylase that catalyses the production of cortisol. Due to lack of this enzyme there is an accumulation of high levels of 17αhydroxyprogesterone (17-OHP). This antibody is therefore recommended for detection of high levels of 17- OHP caused by CAH. Fluorescence enzyme immunoassay and micro-enzyme immunoassay for 17-OHP showed that 4B2.2.3 has lowest cross reactivity with other steroids (PMID: 3312820). |
Antibody First Published | Sawada et al. Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone. J Steroid Biochem. (1987); 28(4):405-10. PMID:3312820 |
Note on publication | Describes the generation of this antibody and its cross reactivity with other steroids. A micro enzyme immunoassay was shown to be applicable for the screening of congenital adrenal hyperplasia. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
