Cat. No.
MABL-3231
Application
inhibit, IP, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NMC-4
From
Recombinant Antibody
Specificity
The NMC-4 antibody binds the GP1b-α binding site on the A1 domain of human von Willebrand factor (also know as vWF or Factor VIII related antigen). vWF is a plasma glycoprotein (GP) that circulates in blood as a series of multimers, in association with factor VIII procoagulant protein. It is produced by endothelial cells and megakaryocytes and is involved in the process of hemostasis by engaging in adhesion of platelets to the sites of vascular injury. Mutations in this gene lead to Von Willebrand's disease which causes frequent bleeding.
Alternative Names
F8VWF; von Willebrand Factor; von Willebrand antigen 2; von Willebrand antigen II; Factor VIII related antigen; NMC4
UniProt
P04275
Immunogen
The original antibody was generated by immunization of BALB/C mice with FVIII/vWF fraction and fusion of spleen cells with NS-1 murine myeloma cells.
Application Notes
The binding characterization of this antibody was done using ELISA (Shima et al, 1985). This antibody was used in the initial characterization of the three distinct functional sites on the von Willebrand factor molecule (PMID: 2477370). This antibody can be used for the immunoprecipitation of the von Willebrand factor from a sample (PMID: 7679102). A study reported that this antibody was shown to inhibit its binding to GP Ib, completely abolished SIPA (sheer induced platelet aggregation) under high shear force, even in the presence of epinephrine. However, this antibody only partially inhibited SIPA under low shear force (PMID: 1360339). This antibody is also reported to completely inhibit vWF binding to platelet glycoprotein (GP) lb induced by either ristocetin or botrocetin at an IgG concentration of approximately 10 micrograms/mL, and also blocked binding of asialo-vWF to GP lb (PMID: 1984791).
Antibody First Published
Shima et al. Production and characterization of monoclonal antibodies against von Willebrand factor (vWF). J. Nura Med. Assoc. (1985), 36,662-669.
Note on publication
Describes the study on various inhibitory actions of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
