Cat. No.
MABL-3230
Application
FACS, ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
F8/86
From
Recombinant Antibody
Specificity
This antibody recognises human von Willebrand factor (also know as Factor VIII related antigen). It is produced by endothelial cells and megakaryocytes and is involved in the process of hemostasis by engaging in adhesion of platelets to the sites of vascular injury. Mutations in this gene lead to Von Willebrand's disease which causes frequent bleeding.
Alternative Names
von Willebrand factor; von Willebrand antigen 2; von Willebrand antigen II; coagulation factor VIII VWF; factor VIII-related antigen; F8; F8VWF; VWD
UniProt
P04275
Immunogen
This antibody was raised against von Willebrand factor isolated from human plasma.
Application Notes
IHC: F8/86 antibody was used in immunohistochemistry expermients to stain against vWF in samples of murine stomach tissue in a study exploring the potential use of mesenchymal cells in myocardium regeneration (Freemen et al., 2015). ICC: This antibody was also used in an attempt to characterise human gingival microvascular endothelial cells where it identified vWF via immunocytochemical staining (DeCarlo et al., 2008). FACS: Another group has successfully used F8/86 antibody in a cell sorting procedure in a study which analysed the influence of physical environment on the rat mesenchymal stem cells (Xue et al., 2017).
Antibody First Published
Naiem et al. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods. 1982;50(2):145-60. PMID:6806388
Note on publication
This article describes generation of the anti-vWF antibody and its characterisation by immunohistological labelling.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
