Anti-CD153 [RM153](MABL-3474)

Cat. No.

MABL-3474

Name

Anti-CD153 [RM153](MABL-3474)

Clone No.

RM153

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, functional assays, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD153, a 40-kDa type II transmembrane protein member of the TNF superfamily.   Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation.

Alternative Name

CD30-L; CD30 ligand; Tumor necrosis factor   ligand superfamily member 8.

UniProt

P32972

Immunogen

This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD153   and fusing the splenocytes with P3U1 myeloma cells.

Application Notes

This antibody has been used in various FACS   analyses, for instance, to demonstrate that lymphoid tissue inducer-like   cells are an innate source of IL-17 and IL-22 (Takatori et al, 2009), and to   suggest that CD30 ligand/CD30 plays a critical role in Th17 differentiation   in mice (Sun et al, 2010). Importantly, the pharmacological effects of this   rat anti-mouse CD153 mAb (clone RM153; IgG2b isotype) have been reported in a   number of settings. For example, this antibody has been shown to prevent or   delay the spontaneous development of diabetes in non-obese diabetic mice when   treatment is initiated in young mice (Chakrabarty et al, 2003), and to   prolong the survival of mice in a model of CD4+ T-cell-mediated lethal   graft-versus-host disease induced by injection of C57BL/6 cells into MHC   class II-only disparate bm12 recipients (Blazar et al, 2004). This antibody   has also been used as parts of in vivo functional assays to demonstrate that   early CD30 signaling is critical for Treg-mediated acute graft-versus-host disease protection after major MHC-mismatch bone marrow transplantation (Zeiser et   al, 2007), and to provide direct evidence that pathogenic memory T cells are   amenable to suppression in an antigen-specific manner while identifying CD30   as a molecule that is critical for the regulation of memory T cell responses   (Dai et al, 2004).

Antibody First   Published

Shimozato et al. Expression of CD30 ligand (CD153) on murine activated T cells. Biochem Biophys Res Commun. 1999 Mar 24;256(3):519-26. PMID:10080930

Note on publication

Describe the original generation and characterisation of this antibody, which was subsequently used in FACS   analyses to confirm the expression of CD153 on murine activated T cells. This   RM153 mAb was shown to bind to mCD153/CHO, mCD153/L5178Y, and mCD153/P815 cells but not to the parental CHO, L5178Y, and P815 cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.