Anti-CD155 [3F1](MABL-3475)

Cat. No.

MABL-3475

Name

Anti-CD155 [3F1](MABL-3475)

Clone No.

3F1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD155.

Alternative Name

TAGE 3F1; Poliovirus receptor; cellular receptor for poliovirus; Pvr; Tumor-associated antigen 1; Taa1; Tage4; HVED

UniProt

Q8K094

Immunogen

This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.

Application Notes

The binding specificity of this antibody has   been confirmed both in ELISA analysis using recombinant murine CD155   (mCD155), and in flow cytometric analysis using HEK293 cells transiently expressing mCD155 (Maier et al, 2007). This antibody has been used in flow cytometry to assess mCD155 expression on lymphocytes and dendritic cells   (Maier et al, 2007; Seth et al, 2011). This antibody blocks the interaction of CD155 with its ligands CD96 and CD226 (Qiu et al, 2010). When administered to WT mice, antibody-induced disruption of the CD155-CD226 interaction has   been shown to cause CD226 upregulation on CD4+ T cells and CD8+ T cells (Seth   et al, 2011). Administration of this antibody has also been shown to stimulate the loss of mature, memory-like CD8+ T cells from the thymus (Seth et al, 2011; Georgiev et al, 2016).

Antibody First   Published

Maier et al. The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested  antigens Eur J Immunol. 2007 Aug;37(8):2214-25. PMID:17621371

Note on publication

Describes the original generation of this antibody, as part of a panel of anti-CD155 antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.