Anti-Galectin 9 [RG9-35](MABL-3473)

Cat. No.

MABL-3473

Name

Anti-Galectin 9   [RG9-35](MABL-3473)

Clone No.

RG9-35

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, functional assays, IP, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine   galectin-9, a 40 kDa S-type lectin that is expressed by various cell types,   including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes,   mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial   cells. Galectin-9 binds to β-galactosides and can serve as a ligand for TIM-3   (CD366). The protein is implicated in both innate and adaptive immune   responses, specifically, induction of cytokine secretion by macrophages,   bactericidal functions, promotion of dendritic cell maturation, regulatory T   cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T   cells.

Alternative Name

ecalectin; Gal-9; galectin 9; Galectin-9; HUAT; lectin, galactose binding, soluble 5; LGALS9A; tumor antigen HOM-HD-21; urate transporter/channel protein.

UniProt

O08573

Immunogen

This antibody was raised by immunising rats   with recombinant mouse galectin-9.

Application Notes

When this antibody was first generated and characterised, it was shown to bind with galectin-9 (Gal-9) but not with either galectin-1 or galectin-3. Furthermore, it was shown to be capable of blocking Tim-3-Fc binding to Gal-9, and inhibiting Gal-9-induced Th1 cell death in vitro (Fukushima et al., 2008; PMID: 18087160). This antibody has been used in FACS   analyses. For instance, to describe a novel mechanism where Tim-3 binds   HMGB-1 on virus-specific CD8+ Treg cells, suppressing proliferation of CD8+ T effector cells during acute adenoviral infection (Dolina et al., 2014; PMID:  24677194); and to indicate a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation (Nakayama et al., 2009; PMID: 19224762). In addition, this antibody has been used in vivo to study the roles of Gal-9 in the development   of experimental allergic conjunctivitis in mice (Fukushima et al., 2008; PMID: 18087160) and to demonstrate that constitutive expression of Gal-9 plays an immunosuppressive role in corneal allografts (Shimmura-Tomita et al., 2013; PMID: 23667648).

Antibody First   Published

Fukushima et al. Roles of galectin-9 in the   development of experimental allergic conjunctivitis in mice. Int Arch Allergy   Immunol. 2008;146(1):36-43. PMID:18087160

Note on publication

Describe the original generation of this   antibody and its subsequent use in in vitro and in vivo functional assays to   study the roles of galectin-9 in the development of experimental allergic   conjunctivitis in mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.