Anti-alpha-2 integrin inserted-domain mAbs

Cat. No.

MABL-372

Name

Anti-alpha-2 integrin inserted-domain mAbs

Clone No.

AFD-2E10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Species Reactivity

Human

Basic Information

Specificity

2E10 was generated by immunizing BALB/c mice with a DNA vaccine containing the humanalpha2 I-domain cDNAsequence within pVAC2. The alpha2 I-domain is expressed as a fusion protein withfragment C of tetanus toxoid (FrC-TT). The scFv was then created using a V-gene phage display library derived from RNA from mice splenocytes and isolated following selection on GST-I after 3 rounds of panning.

Alternative Name

CD49b; alpha2-beta1 integrin; glycoprotein IaIIa; GP IaIIa; VLA2; VLA-2;

UniProt

Immunogen

Human

Application Notes

scFv 2E10 can bind to the I (inserted)   domain of recombinant alpha2 (GST- and His-), but cannot bind to the native epitope on platelets. This scFv also cross-reacts with and binds to the   I-domain of alpha1 integrin. The alpha2 I-domain consists of ~200 amino acids   and contains the ligand-binding site. The α2β1 integrin is expressed on   platelets, fibroblasts, activated T lympho-cytes, endothelial cells and   epithelial cells where its function is to act as a collagen and laminin  receptor. 2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain   in ELISA and FC studies.

Antibody First Published

Hughes et al. Platelet integrin alpha2 I-domain specific antibodies produced via domain specific DNA vaccination combined with variable gene phage display. Thromb Haemost. 2005Dec;94(6) 1318-26. PMID16411412

Note on publication

Describes the generation of mice scFvs by   phage display and rabbit polyclonal antibodies raised against alpha2 intergin   I-domain. The scFv specificity and reactivity was determined by ELISA and FC.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.