Key features and details | |
Cat. No. | MABL-370 |
Name | Anti-alpha V beta 6 integrin mAbs |
Clone No. | AFD- EM05201 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the extracellular domain of human recombinant transmembranetruncated (DTM) αvβ6 integrin, a dimeric cell surface protein that mediates cell attachment. αvβ6 integrin promotes the activation of latent TGF-β. |
Alternative Name | αvβ6; Vitronectin receptor subunit alpha; CD51; ITGB6; Integrin beta-6; P06756; P18564; avb6 |
UniProt | |
Immunogen | This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas |
Application Notes | This antibody has been used to specifically bind αvβ6 in Western blots and in flow cytometry, and to specifically stain αvβ6 integrins in FFPE (formalin fixed paraffin embedded)/TMA material, where murine antibodies function poorly (Goodman et al, 2012; Heß et al., 2014; Roth et al, 2013). This revealed αvβ6 to be strongly expressed in samples of invasive ductal breast adenoma, colorectal carcinoma, and NSCLC. |
Antibody First Published | Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423 |
Note on publication | Describes the generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
