Anti-Acetyllysine mAbs

Cat. No.

MABL-350

Name

Anti-Acetyllysine mAbs

Clone No.

AFD- ACK2F12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, WB

Species Reactivity

Species independent

Basic Information

Specificity

This antibody recognizes acetly lysine and  more particularly Nε-acetyllysine.

Alternative Name

acetylated lysine; acetyl derivative of amino acid lysine; N-ε-acetyl-Llysine

UniProt

Immunogen

The original antibody was generated by immunizing mice with chemically acetylated keyhole limpet hemocyanin.

Application Notes

This antibody is capable of   identifying acetylated BSA and various acetlylysine containing mouse peptides in an ELISA. This antibody can also recognize acetyllysine-containing protein from human T cell leukemia cell line MOLT-4F lysate in a western blot assay   (EP1783142, ISBN-10: 1594545502). The HRP-SH conjugated ACK2F12 was capable   of detecting chemically conjugated BSA in a western blot assay (EP1783142).   This antibody was used in the study of organ specific post translational   lyine acetylation in mice (PMID: 16247734)

Antibody First Published

Iwabata et al. Proteomic analysis of   organ-specific post-translational lysineacetylation and -methylation in mice   by use of anti-acetyllysine and -methyllysine mouse monoclonal antibodies. Proteomics. (2005);  5(18):4653-64.   PMID:1624773

Note on publication

Describes the analysis of organ-specific post-translational lysine-acetylation in mice using this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.