Key features and details | |
Cat. No. | MABL-349 |
Name | Anti-Acetylcholinesterase mAbs |
Clone No. | AFD- Elec408 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, inhibition, RIA, SPR |
Species Reactivity | Electrophorus electricus |
Basic Information | |
Specificity | The antibody is specific for EeAChE, and it targets an epitope located in the backdoor region, distant from the gorge entrance. The antibody does not cross react with human AChE, bovine AChE, Torpedo AChE, and snake AChE or denatured AChE. The antibody binds the EeAChE subunit noncompetitively with PAS ligands Fas2, Elec403 and Elec410. |
Alternative Name | AChE; EC 3.1.1.7 |
UniProt | O42275 |
Immunogen | The original antibody was generated by immunizing mice against Electrophorus electricus acetylcholinesterase. |
Application Notes | The original antibody (IgG1) bound AChE with high affinity and strongly inhibited the antigen with an apparent Ki value less than 0.1 nM. The relative affinity of the antibody for AChE was evaluated using a competitive radioimmunoassay (Kd= 38 pM). The inhibition of E. electricus AChE by the antibody was studied as a function of substrate concentration, the inhibition obtained with the antibody was about 75 % (Remy et al., 1995; PMID: 7649165). The epitope mapping was studied by site-directed mutagenesis, showing the antibody binds to the AChE back door (Simon et al., 1999; PMID: 10488117). The structure of the Fab fragment was determined by X-ray (Bourne et al., 2013; PMID: 24146971). |
Antibody First Published | Remy et al. Characterization of monoclonal antibodies that strongly inhibit Electrophorus electricus acetylcholinesterase. Eur J Biochem 231: 651-658. PMID:7649165 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
